{"created":"2023-06-19T07:03:00.514120+00:00","id":5213,"links":{},"metadata":{"_buckets":{"deposit":"01082b11-25a9-41da-a0a1-a4df83a76f20"},"_deposit":{"created_by":3,"id":"5213","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5213"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00005213","sets":["9:27:28:29"]},"author_link":["16322","4684","16324","4683","16319","16320","16327","16328","16323","16326","16325","16321"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-06","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"1021-12","bibliographicPageStart":"1021-1","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"Frontiers in Microbiology"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The emergence of antimicrobial resistance in Klebsiella spp., including resistance to extended-spectrum cephalosporins (ESC) and fluoroquinolones, is of great concern in both human and veterinary medicine. In this study, we investigated the prevalence of antimicrobial resistance in a total of 103 Klebsiella spp. isolates, consisting of Klebsiella pneumoniae complex (KP, n = 89) and K. oxytoca (KO, n = 14) from clinical specimens of dogs and cats in Japan. Furthermore, we characterized the resistance mechanisms, including extended-spectrum β-lactamase (ESBL), plasmid-mediated AmpC β-lactamase (PABL), and plasmid-mediated quinolone resistance (PMQR); and assessed genetic relatedness of ESC-resistant Klebsiella spp. strains by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Antimicrobial susceptibility testing demonstrated that resistance rates to ampicillin, cephalothin, enrofloxacin, ciprofloxacin, trimethoprim/sulfamethoxazole, cefotaxime, gentamicin, tetracycline, chloramphenicol, amoxicillin-clavulanic acid, and cefmetazole were 98.1, 37.9, 37.9, 35.9, 35.0, 34.0, 31.1, 30.1, 28.2, 14.6, and 6.8%, respectively. Phenotypic testing detected ESBLs and/or AmpC β-lactamases in 31 of 89 (34.8%) KP isolates, but not in KO isolates. Resistances to 5 of the 12 antimicrobials tested, as well as the three PMQRs [qnrB, qnrS, and aac(6′)-Ib-cr], were detected significantly more frequently in ESBL-producing KP, than in non-ESBL-producing KP and KO. The most frequent ESBL was CTX-M-15 (n = 13), followed by CTX-M-14 (n = 7), CTX-M-55 (n = 6), SHV-2 (n = 5), CTX-M-2 (n = 2), and CTX-M-3 (n = 2). Based on the rpoB phylogeny, all ESBL-producing strains were identified as K. pneumoniae, except for one CTX-M-14-producing strain, which was identified as K. quasipneumoniae. All of AmpC β-lactamase positive isolates (n = 6) harbored DHA-1, one of the PABLs. Based on MLST and PFGE analysis, ST15 KP clones producing CTX-M-2, CTX-M-15, CTX-M-55, and/or SHV-2, as well as KP clones of ST1844-CTX-M-55, ST655-CTX-M-14, and ST307-CTX-M-15, were detected in one or several hospitals. Surprisingly, specific clones were detected in different patients at an interval of many months. These results suggest that multidrug-resistant ESBL-producing KP were clonally disseminated among companion animals via not only direct but also indirect transmission. This is the first report on large-scale monitoring of antimicrobial-resistant Klebsiella spp. isolates from companion animals in Japan.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Frontiers Media S.A."}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.3389/fmicb.2016.01021","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2016 Harada, Shimizu, Mukai, Kuwajima, Sato, Usui, Tamura, Kimura, Miyamoto, Tsuyuki, Ohki and Kataoka. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms."}]},"item_2_rights_16":{"attribute_name":"権利（URI）","attribute_value_mlt":[{"subitem_rights":"https://creativecommons.org/licenses/by/4.0/ | https://creativecommons.org/licenses/by/4.0/"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Harada, Kazuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shimizu, Takae"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mukai, Yujiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuwajima, Ken"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sato, Tomomi"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"USUI, Masaru","creatorNameLang":"en"},{"creatorName":"臼井, 優","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Tamura, Yutaka"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kimura, Yui"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Miyamoto, Tadashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsuyuki, Yuzo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohki, Asami"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kataoka, Yasushi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-12"}],"displaytype":"detail","filename":"R-2017-238_Usui.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"R-2017-238_Usui.pdf","url":"https://rakuno.repo.nii.ac.jp/record/5213/files/R-2017-238_Usui.pdf"},"version_id":"5c6cda68-6434-4d7e-acde-dc998cc4db38"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Klebsiella spp.","subitem_subject_scheme":"Other"},{"subitem_subject":"dogs","subitem_subject_scheme":"Other"},{"subitem_subject":"cats","subitem_subject_scheme":"Other"},{"subitem_subject":"extended-spectrum b-lactamases","subitem_subject_scheme":"Other"},{"subitem_subject":"multidrugresistance","subitem_subject_scheme":"Other"},{"subitem_subject":"clonal dissemination","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Phenotypic and Molecular Characterization of Antimicrobial Resistance in Klebsiella spp. Isolates from Companion Animals in Japan : Clonal Dissemination of Multidrug-Resistant Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Phenotypic and Molecular Characterization of Antimicrobial Resistance in Klebsiella spp. Isolates from Companion Animals in Japan : Clonal Dissemination of Multidrug-Resistant Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-04-12"},"publish_date":"2018-04-12","publish_status":"0","recid":"5213","relation_version_is_last":true,"title":["Phenotypic and Molecular Characterization of Antimicrobial Resistance in Klebsiella spp. Isolates from Companion Animals in Japan : Clonal Dissemination of Multidrug-Resistant Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-31T01:58:09.515232+00:00"}