{"created":"2023-06-19T07:03:00.770163+00:00","id":5217,"links":{},"metadata":{"_buckets":{"deposit":"5f3aab27-f217-43c9-b2e3-f4bb74b88701"},"_deposit":{"created_by":3,"id":"5217","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5217"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00005217","sets":["9:10:18:19"]},"author_link":["16362","16354","16358","3503","16353","16363","16357","16364","16360","16355","16359","16361","16356"],"item_2_alternative_title_22":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Identification of Mushroom Species by Automated rRNA Intergenic Spacer Analysis（ARISA） and Its Application to a Suspected Case of Food Poisoning with Tricholoma ustale"}]},"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"45","bibliographicPageStart":"37","bibliographicVolumeNumber":"57","bibliographic_titles":[{"bibliographic_title":"食品衛生学雑誌 = Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Automated rRNA intergenic spacer analysis (ARISA), a method of microbiome analysis, was evaluated for species identification of mushrooms based on the specific fragment sizes. We used 51 wild mushroom-fruiting bodies collected in the centre of Hokkaido and two cultivated mushrooms. Samples were hot-air-dried and DNA were extracted by a beads beating procedure. Sequencing analysis of portions of the rRNA gene (rDNA) provided 33 identifications of mushrooms by genus or species. The results of ARISA identification based on the combination of the fragment sizes corresponding to two inter spacer regions (ITS2 and ITS1) of rDNA within±0.1% accuracy showed that 27 out of the 33 species had specific fragment sizes differentiated from other species. The remaining 6 species formed 3 pairs that showed overlapping fragment sizes. In addition, within-species polymorphisms were observed as 1 bp differences among 32 samples of 13 species. ARISA was applied to investigate a case of suspected food poisoning in which the mushroom was thought to be a toxic Kakishimeji. The morphological identification of the mushroom was ambiguous since the remaining sample lacked a part of the fruiting body. Further, yeast colonies had grown on the surface of the fruiting body during storage. The ARISA fragment size of the mushroom showed 7 bp difference from that of the candidate toxic mushroom. Although ARISA could be a useful tools for estimation of mushroom species, especially in case where the fruiting bodies have deteriorated or been processed, further studies are necessary for reliable identification. For example, it may be necessary to adopt more informative genes which could provide clearer species-specific polymorphisms than the ITS regions.","subitem_description_type":"Abstract"}]},"item_2_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"16359","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Sugawara, Ryota"}]},{"nameIdentifiers":[{"nameIdentifier":"16360","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Yamada, Sayumi"}]},{"nameIdentifiers":[{"nameIdentifier":"16361","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Tu, Zhihao"}]},{"nameIdentifiers":[{"nameIdentifier":"16362","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Sugawara, Akiko"}]},{"nameIdentifiers":[{"nameIdentifier":"16363","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Hoshiba, Toshihiro"}]},{"nameIdentifiers":[{"nameIdentifier":"16364","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Eisaka, Sadao"}]},{"nameIdentifiers":[{"nameIdentifier":"3503","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"1d477d35097aa45360392a0d922b9077","nameIdentifierScheme":"教員総覧","nameIdentifierURI":"http://souran.rakuno.ac.jp/profile/ja.1d477d35097aa45360392a0d922b9077.html"}],"names":[{"name":"Yamaguchi, Akihiro"}]}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本食品衛生学会"}]},"item_2_relation_11":{"attribute_name":"ISBN","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"0015-6426","subitem_relation_type_select":"ISBN"}}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.3358/shokueishi.57.37","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"この論文は「食品衛生学雑誌」に掲載されたものであり、著作権のある（公社）日本食品衛生学会 の許諾を受けて公開しております。"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"菅原, 諒太"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山田, さゆみ"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"涂, 志豪"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"菅原, 明子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"干場, 敏博"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"栄坂, 貞夫"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山口, 昭弘"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-12"}],"displaytype":"detail","filename":"R-2017-249_Yamaguchi.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"R-2017-249_Yamaguchi.pdf","url":"https://rakuno.repo.nii.ac.jp/record/5217/files/R-2017-249_Yamaguchi.pdf"},"version_id":"d352effa-852d-4884-9a1a-606256b60ab6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"DNA多型","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA polymorphism","subitem_subject_scheme":"Other"},{"subitem_subject":"品種鑑定","subitem_subject_scheme":"Other"},{"subitem_subject":"species identification","subitem_subject_scheme":"Other"},{"subitem_subject":"キノコ","subitem_subject_scheme":"Other"},{"subitem_subject":"mushroom","subitem_subject_scheme":"Other"},{"subitem_subject":"自然毒","subitem_subject_scheme":"Other"},{"subitem_subject":"natural toxin","subitem_subject_scheme":"Other"},{"subitem_subject":"食中毒","subitem_subject_scheme":"Other"},{"subitem_subject":"food poisoning","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"自動rRNA遺伝子間多型解析（ARISA）によるキノコ種鑑別の検討とカキシメジによる食中毒疑い事例への適用の試み","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"自動rRNA遺伝子間多型解析（ARISA）によるキノコ種鑑別の検討とカキシメジによる食中毒疑い事例への適用の試み"}]},"item_type_id":"2","owner":"3","path":["19"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-04-12"},"publish_date":"2018-04-12","publish_status":"0","recid":"5217","relation_version_is_last":true,"title":["自動rRNA遺伝子間多型解析（ARISA）によるキノコ種鑑別の検討とカキシメジによる食中毒疑い事例への適用の試み"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-19T08:05:39.752287+00:00"}