{"created":"2023-06-19T07:03:22.178891+00:00","id":5644,"links":{},"metadata":{"_buckets":{"deposit":"b26debda-103e-4177-b185-62004d693554"},"_deposit":{"created_by":3,"id":"5644","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5644"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00005644","sets":["9:27:28:29"]},"author_link":["17713","17708","4871","17711","17717","17712","17707","17716","17718","17715","17714","17709","4494","17719","17710"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-09","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"363","bibliographicPageStart":"355","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"Immunity, Inflammation and Disease"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"【Introduction】 Bovine mycoplasma, chiefly Mycoplasma bovis, is a pathogen that causes pneumonia, mastitis, arthritis, and otitis media in cattle. This pathogen exerts immunosuppressive effects, such as the inhibition of interferon production. However, the mechanisms involved in bovine mycoplasmosis have not been fully elucidated. In this study, we investigated the role of the programmed death‐1 (PD‐1)/programmed death‐ligand 1 (PD‐L1) pathway in immunosuppression in bovine mycoplasmosis. 【Methods】 In the initial experiments, we used enzyme‐linked immunosorbent assay to measure interferon‐γ (IFN‐γ) from peripheral blood mononuclear cells (PBMCs) isolated from cattle with mycoplasmosis. 【Results】 Expectedly, IFN‐γ production significantly decreased in cattle with mycoplasmosis compared with that in clinically healthy cattle. Concomitantly, flow cytometric analysis revealed that the proportions of PD‐1^+CD4^+ and PD‐L1^+CD14^+ cells significantly increased in peripheral blood of the infected cattle. Interestingly, the number of PD‐1^+CD4^+ and PD‐1^+CD8^+ T cells were negatively correlated with IFN‐γ production from PBMCs in bovine mycoplasmosis. Additionally, blockade of the PD‐1/PD‐L1 pathway in vitro by anti‐bovine PD‐1‐ and anti‐bovine PD‐L1 antibodies significantly upregulated the production of IFN‐γ from anti‐mycoplasma‐specific cells. 【Conclusions】 These results suggest that the PD‐1/PD‐L1 pathway could be involved in immune exhaustion of bovine mycoplasma‐specific T cells. In conclusion, our study opens up a new perspective in the therapeutic strategy for bovine mycoplasmosis by targeting the immunoinhibitory receptor pathways.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"John Wiley & Sons Ltd"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1002/iid3.173","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2017 The Authors. Immunity, Inflammation and Disease Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited."}]},"item_2_rights_16":{"attribute_name":"権利（URI）","attribute_value_mlt":[{"subitem_rights":"https://creativecommons.org/licenses/by/4.0/ | https://creativecommons.org/licenses/by/4.0/"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Goto, Shinya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Konnai, Satoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okagawa, Tomohiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishimori, Asami"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maekawa, Naoya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Gondaira, Satoshi"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"樋口, 豪紀","creatorNameLang":""}],"familyNames":[{"familyNameLang":""}],"givenNames":[{"givenNameLang":""}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Koiwa, Masateru"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"田島, 誉士","creatorNameLang":""}],"familyNames":[{"familyNameLang":""}],"givenNames":[{"givenNameLang":""}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kohara, Junko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ogasawara, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Yukinari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Yasuhiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Murata, Shiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohashi, Kazuhiko"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-08"}],"displaytype":"detail","filename":"R-2018-18_tajima.pdf","filesize":[{"value":"430.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"R-2018-18_tajima.pdf","url":"https://rakuno.repo.nii.ac.jp/record/5644/files/R-2018-18_tajima.pdf"},"version_id":"429cdac5-ed8a-4442-aade-ee26e8f7616f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Bovine mycoplasmosis","subitem_subject_scheme":"Other"},{"subitem_subject":"IFN-g","subitem_subject_scheme":"Other"},{"subitem_subject":"immunosuppression","subitem_subject_scheme":"Other"},{"subitem_subject":"PD-1","subitem_subject_scheme":"Other"},{"subitem_subject":"PD-L1","subitem_subject_scheme":"Other"},{"subitem_subject":"Bovine mycoplasmosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"IFN-g","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"immunosuppression","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PD-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PD-L1","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Increase of cells expressing PD‐1 and PD‐L1 and enhancement of IFN‐γ production via PD‐1/PD‐L1 blockade in bovine mycoplasmosis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Increase of cells expressing PD‐1 and PD‐L1 and enhancement of IFN‐γ production via PD‐1/PD‐L1 blockade in bovine mycoplasmosis"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-08-08"},"publish_date":"2019-08-08","publish_status":"0","recid":"5644","relation_version_is_last":true,"title":["Increase of cells expressing PD‐1 and PD‐L1 and enhancement of IFN‐γ production via PD‐1/PD‐L1 blockade in bovine mycoplasmosis"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-31T01:20:00.478385+00:00"}