{"created":"2023-06-19T07:03:22.437295+00:00","id":5650,"links":{},"metadata":{"_buckets":{"deposit":"fdb8e4e1-3209-470a-9690-d74060426f76"},"_deposit":{"created_by":3,"id":"5650","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5650"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00005650","sets":["9:10:18:19"]},"author_link":["17774","17775","17777","17780","17779","17781","17778","17776"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"228","bibliographicPageStart":"217","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"Journal of innate immunity"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Collectins are C-type lectins that are involved in innate immunity as pattern recognition molecules. Recently, collectin kidney 1 (CL-K1) has been discovered, and in vitro studies have shown that CL-K1 binds to microbes and activates the lectin complement pathway. However, in vivo functions of CL-K1 against microbes have not been elucidated. To investigate the biological functions of CL-K1, we generated CL-K1 knockout (CL-K1-/-) mice and then performed a Streptococcus pneumoniae infection analysis. First, we found that recombinant human CL-K1 bound to S. pneumoniae in a calcium-dependent manner, and induced complement activation. CL-K1-/- mice sera formed less C3 deposition on S. pneumoniae. Furthermore, immunofluorescence analysis in the wild-type (WT) mice demonstrated that CL-K1 and C3 were localized on S. pneumoniae in infected lungs. CL-K1-/- mice revealed decreased phagocytosis of S. pneumoniae. Consequently, less S. pneumoniae clearance was observed in their lungs. CL-K1-/- mice showed severe pulmonary inflammation and weight loss in comparison with WT mice. Finally, the decreased clearance and severe pulmonary inflammation caused by S. pneumoniae infection might cause higher CL-K1-/- mice lethality. Our results suggest that CL-K1 might play an important role in host protection against S. pneumoniae infection through the activation of the lectin complement pathway.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Karger Publishers"}]},"item_2_relation_11":{"attribute_name":"ISBN","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"1662-811X","subitem_relation_type_select":"ISBN"}}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1159/000453316","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2017 S. Karger AG, Basel"},{"subitem_rights":"The final,published version of this article is available at https://www.karger.com/Article/FullText/453316"}]},"item_2_rights_16":{"attribute_name":"権利（URI）","attribute_value_mlt":[{"subitem_rights":"https://creativecommons.org/licenses/by-nc-nd/4.0/ | https://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hwang, Insu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mori, Kenichiro"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Ohtani, Katsuki","creatorNameLang":"en"},{"creatorName":"大谷, 克城","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Matsuda, Yasuyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Roy, Nitai"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kim, YounUck"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Yasuhiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wakamiya, Nobutaka"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-08"}],"displaytype":"detail","filename":"R-2018-30_ohtani.pdf","filesize":[{"value":"402.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"R-2018-30_ohtani.pdf","url":"https://rakuno.repo.nii.ac.jp/record/5650/files/R-2018-30_ohtani.pdf"},"version_id":"39545f86-5242-428d-8ca1-c90585555e85"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Collectin","subitem_subject_scheme":"Other"},{"subitem_subject":"Complement","subitem_subject_scheme":"Other"},{"subitem_subject":"Innate immunity","subitem_subject_scheme":"Other"},{"subitem_subject":"Knockout mice","subitem_subject_scheme":"Other"},{"subitem_subject":"Pneumonia","subitem_subject_scheme":"Other"},{"subitem_subject":"Collectin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Complement","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Innate immunity","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Knockout mice","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Pneumonia","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Collectin Kidney 1 Plays an Important Role in Innate Immunity against Streptococcus pneumoniae Infection","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Collectin Kidney 1 Plays an Important Role in Innate Immunity against Streptococcus pneumoniae Infection"}]},"item_type_id":"2","owner":"3","path":["19"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-08-08"},"publish_date":"2019-08-08","publish_status":"0","recid":"5650","relation_version_is_last":true,"title":["Collectin Kidney 1 Plays an Important Role in Innate Immunity against Streptococcus pneumoniae Infection"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-31T00:52:30.973435+00:00"}