@article{oai:rakuno.repo.nii.ac.jp:00005656,
 author = {Shimamori, Toshio and NODA, Jyun and 能田, 淳 and Tsukano, Kenji and Sera, Kouichiro and Yokota, Hiroshi and Koiwa, Masateru and Suzuki, Takahide and 鈴木, 一由},
 issue = {1},
 journal = {Japanese Journal of Veterinary Research},
 month = {Feb},
 note = {Article, The aim of the present study was to examine the applicability of the direct determination of trace and major element concentrations in serum samples collected from Holstein dairy cattle with acute coliform mastitis (n = 53) compared with a healthy control group (n = 39). Twenty-eight elements (Na, Mg, Al, Si, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Ce, Ni, Cu, Zn, Ga, As, Se, Br, Rb, Sr, Y, Zr, Nb, Mo, and Pb) were detected by particle-induced X-ray emission (PIXE). Significant differences were observed in serum K, Fe, Zn, and Br concentrations, but not in those of the remaining twenty-four elements. Furthermore, serum Fe concentrations (0.751 ± 0.583 μg/ml, n = 18) were significantly lower in dairy cattle with a poor prognosis than in those with a good prognosis (0.945 ± 0.393 μg/ml, n = 35, P < 0.05) and healthy controls (1.458 ± 0.391 μg/ml, n = 39, P < 0.01). We proposed a diagnostic cut-off point for serum Fe concentrations of <0.82 μg/ml based on receiver operating characteristic (ROC) curves in order to identify cattle with a poor prognosis. The results of the present study indicated that assessing the elemental composition of serum, particularly iron, is a promising prognostic tool for determining the outcomes of cattle with severe acute coliform mastitis.},
 pages = {29--37},
 title = {Particle-Induced X-ray Emission Analysis of Serum Trace and Major Elements in Cattle with Acute Coliform Mastitis},
 volume = {65},
 year = {2017}
}