@article{oai:rakuno.repo.nii.ac.jp:00005708, author = {SAJIKI, Yamato and KONNAI, Satoru and NISHIMORI, Asami and OKAGAWA, Tomohiro and MAEKAWA, Naoya and GOTO, Shinya and NAGANO, Masashi and KOHARA, Junko and KITANO, Nana and TAKAHASHI, Toshihiko and 田島, 誉士 and MEKATA, Hirohisa and HORII, Yoichiro and MURATA, Shiro and OHASHI, Kazuhiko}, issue = {12}, journal = {The Journal of veterinary medical science}, month = {Dec}, note = {Article, Enzootic bovine leukemia is caused by the bovine leukemia virus (BLV). BLV is transmitted vertically or horizontally through the transfer of infected cells via direct contact, through milk, insect bites and contaminated iatrogenic procedures. However, we lacked direct evidence of intrauterine infection. The purpose of this study was to confirm intrauterine BLV infection in two pregnant dams with high viral load by cesarean delivery. BLV was detected in cord and placental blood, and the BLV in the newborns showed 100% nucleotide identity with the BLV-env sequence from the dams. Notably, a newborn was seropositive for BLV but had no colostral antibodies. In this study, we presented a direct evidence of intrauterine BLV transmission in pregnant dam with a high proviral load. These results could aid the development of BLV control measures targeting viral load.}, pages = {2036--2039}, title = {Intrauterine infection with bovine leukemia virus in pregnant dam with high viral load}, volume = {79}, year = {2017} }