@article{oai:rakuno.repo.nii.ac.jp:00006064,
 author = {Yamashita, Chizuru and Moriyama, Kazuhiro and Hasegawa, Daisuke and Kato, Yu and Sakai, Toshikazu and Kawaji, Takahiro and Shimomura, Yasuyo and Kurimoto, Yasuyoshi and Nagata, Mariko and Nishida, Osamu},
 issue = {4},
 journal = {Blood purification},
 month = {Oct},
 note = {Article, Background: Polymyxin B-immobilized fiber (PMX-F) columns are used as therapeutic interventions for septic shock. The clinical efficacy has been reported for 2-h applications, but their ability to adsorb endotoxin over longer treatments has not been fully elucidated. We hypothesized that PMX-F columns are capable of endotoxin removal for more than 2 h. Method: We designed closed circuits incorporating either a PMX-F column with an 8.5-mL priming volume (PMX-01R) or a sham-control column, and used inactivated fetal bovine serum as the circulating perfusate. Endotoxin was continuously injected at a fixed rate for 24 h, and perfusate endotoxin concentrations were measured at fixed time points. PMX-01R endotoxin adsorption was calculated from the difference in the endotoxin concentrations. Results: PMX-01R endotoxin adsorption increased continuously in a virtually linear manner. Conclusions: The PMX-01R column showed sustained endotoxin adsorption for at least 24 h. This indicated that PMX-F columns would be capable of clinical endotoxin removal for 24 h.},
 pages = {269--273},
 title = {In Vitro Study of Endotoxin Adsorption by a Polymyxin B-Immobilized Fiber Column},
 volume = {46},
 year = {2018}
}