{"created":"2023-06-19T07:03:40.561479+00:00","id":6076,"links":{},"metadata":{"_buckets":{"deposit":"82d7236c-4c2e-4015-9872-610a8592ecc4"},"_deposit":{"created_by":3,"id":"6076","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"6076"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00006076","sets":["9:27:28:29"]},"author_link":["19429","19430","19433","19432","4859","4494","19427","4860","19431","19428"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-04","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"13","bibliographicPageStart":"6","bibliographicVolumeNumber":"198","bibliographic_titles":[{"bibliographic_title":"Veterinary Immunology and Immunopathology"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The scavenger receptor CD163 is widely used as a cell signature for alternatively active “M2” macrophages in mammals. In this study, we generated two monoclonal antibodies, ABM-1A9 and ABM-2D6, against the extracellular region of bovine CD163. Conventional Western blotting using the antibodies yielded immunoreactive bands of bovine CD163 at 130 and 150 kDa in non-reduced and reduced spleen lysates, respectively. The minimum limit of detectable concentration of both antibodies was relatively lower (5.0 ng/mL) than that of the anti-human CD163 monoclonal antibody AM–3 K (>1.0 μg/mL), which has been used previously for the detection of bovine CD163. An immunohistochemical study using formalin-fixed paraffin-embedded sections revealed that ABM-1A9 and ABM-2D6 clearly stained some Iba1^+ macrophages in the lymph nodes of cattle with mastitis. Moreover, the CD163-stained macrophages were frequently observed engulfing leukocytes. ELISA using ABM-2D6 distinguished levels of circulating soluble CD163 in healthy cattle (less than 16.9 pmol/mL) and cattle with mastitis (more than 33.7 pmol/mL). These new monoclonal antibodies can be used in the diagnosis and evaluation of inflammatory disease prognosis in cattle with immunohistological analyses and blood test applications.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V."}]},"item_2_relation_11":{"attribute_name":"ISBN","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"0165-2427","subitem_relation_type_select":"ISBN"}}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.vetimm.2018.02.004","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license."}]},"item_2_rights_16":{"attribute_name":"権利（URI）","attribute_value_mlt":[{"subitem_rights":"http://creativecommons.org/licenses/by-nc-nd/4.0/ | http://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shimamoto, Yoshinori"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Nio-Kobayashi, Junko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watarai, Hiroshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nagano, Masashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saito, Natsuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takahashi, Eiki"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"樋口, 豪紀","creatorNameLang":""}],"familyNames":[{"familyNameLang":""}],"givenNames":[{"givenNameLang":""}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kobayashi, Atsushi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kimura, Takashi"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"KITAMURA, Hiroshi","creatorNameLang":"en"},{"creatorName":"北村, 浩","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-26"}],"displaytype":"detail","filename":"R-2019-45_kitamura.pdf","filesize":[{"value":"7.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"R-2019-45_kitamura.pdf","url":"https://rakuno.repo.nii.ac.jp/record/6076/files/R-2019-45_kitamura.pdf"},"version_id":"0959e0da-9eb6-40f8-b0f8-29bc91c71ef9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"CD163","subitem_subject_scheme":"Other"},{"subitem_subject":"M2 macrophage","subitem_subject_scheme":"Other"},{"subitem_subject":"Monoclonal antibody","subitem_subject_scheme":"Other"},{"subitem_subject":"Inflammation","subitem_subject_scheme":"Other"},{"subitem_subject":"Immunohistochemistry","subitem_subject_scheme":"Other"},{"subitem_subject":"ELISA","subitem_subject_scheme":"Other"},{"subitem_subject":"CD163","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"M2 macrophage","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Monoclonal antibody","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Inflammation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Immunohistochemistry","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ELISA","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Generation and validation of novel anti-bovine CD163 monoclonal antibodies ABM-1A9 and ABM-2D6","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Generation and validation of novel anti-bovine CD163 monoclonal antibodies ABM-1A9 and ABM-2D6"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-02-26"},"publish_date":"2020-02-26","publish_status":"0","recid":"6076","relation_version_is_last":true,"title":["Generation and validation of novel anti-bovine CD163 monoclonal antibodies ABM-1A9 and ABM-2D6"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-31T01:35:37.354023+00:00"}