{"created":"2023-06-19T07:03:42.005853+00:00","id":6110,"links":{},"metadata":{"_buckets":{"deposit":"c2abd395-7204-455a-a826-d85810e88210"},"_deposit":{"created_by":3,"id":"6110","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"6110"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00006110","sets":["9:27:33:34"]},"author_link":["6295","19581","5859","19582"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"81","bibliographicPageStart":"70","bibliographicVolumeNumber":"99","bibliographic_titles":[{"bibliographic_title":"Peptides"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We identified guinea pig ghrelin (gp-ghrelin), and examined its distribution and physiological actions in the guinea-pig. Gp-ghrelin is a 28-amino acid peptide (GASFR SPEHH SAQQR KESRK LPAKI QPR); seven amino acids are different from that of rat ghrelin at positions 2, 5, 10, 11, 19, 21, and 25, which include the conserved region known in mammals. The third serine residue is mainly modified by n-decanoyl acid. Both gp-ghrelin and rat ghrelin increased intracellular Ca^<2+> concentration of HEK293 cells expressing guinea pig growth hormone secretagogue receptor 1a (GHS-R1a), and the affinity of gp-ghrelin was slightly higher than that of rat ghrelin. In addition, gp-ghrelin was also effective in CHO cells expressing rat GHS-R1a with similar affinity to that of rat ghrelin. Gp-ghrelin mRNA was predominantly expressed in the stomach, whereas the expression levels in other organs was low. High levels of GHS-R1a mRNA expression were observed in the pituitary, medulla oblongata, and kidney, while medium levels were noted in the thalamus, pons, olfactory bulb, and heart. Immunohistochemistry identified gp-ghrelin-immunopositive cells in the gastric mucosa and pancreas. Intraperitoneal injection of gp-ghrelin increased food intake in the guinea pig. Gp-ghrelin did not cause any mechanical responses in isolated gastrointestinal smooth muscles in vitro, similar to rat ghrelin. In conclusion, the N-terminal structures that are conserved in mammals were different in gp-ghrelin. Moreover, the functional characteristics of gp-ghrelin, other than its distribution, were dissimilar from those in other Rodentia.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier Inc."}]},"item_2_relation_11":{"attribute_name":"ISBN","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"0196-9781","subitem_relation_type_select":"ISBN"}}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.peptides.2017.11.010","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2017 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license."}]},"item_2_rights_16":{"attribute_name":"権利（URI）","attribute_value_mlt":[{"subitem_rights":"http://creativecommons.org/licenses/by-nc-nd/4.0/ | http://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Okuhara, Yuji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kaiya, Hiroyuki"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"TERAOKA, Hiroki","creatorNameLang":""}],"familyNames":[{"familyNameLang":""}],"givenNames":[{"givenNameLang":""}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kitazawa, Takio"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-26"}],"displaytype":"detail","filename":"R-2019-143_kitazawa.pdf","filesize":[{"value":"2.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"R-2019-143_kitazawa.pdf","url":"https://rakuno.repo.nii.ac.jp/record/6110/files/R-2019-143_kitazawa.pdf"},"version_id":"03958aa6-4776-4579-ade7-6da47318569d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Guinea pig","subitem_subject_scheme":"Other"},{"subitem_subject":"Ghrelin","subitem_subject_scheme":"Other"},{"subitem_subject":"Growth hormone secretagogue receptor 1a (GHS-R1a)","subitem_subject_scheme":"Other"},{"subitem_subject":"cDNA cloning","subitem_subject_scheme":"Other"},{"subitem_subject":"Functional characterization","subitem_subject_scheme":"Other"},{"subitem_subject":"Tissue distribution","subitem_subject_scheme":"Other"},{"subitem_subject":"Guinea pig","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Ghrelin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Growth hormone secretagogue receptor 1a (GHS-R1a)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cDNA cloning","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Functional characterization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Tissue distribution","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Structural determination, distribution, and physiological actions of ghrelin in the guinea pig","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Structural determination, distribution, and physiological actions of ghrelin in the guinea pig"}]},"item_type_id":"2","owner":"3","path":["34"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-02-26"},"publish_date":"2020-02-26","publish_status":"0","recid":"6110","relation_version_is_last":true,"title":["Structural determination, distribution, and physiological actions of ghrelin in the guinea pig"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-31T02:22:53.773571+00:00"}