{"created":"2023-06-19T07:03:54.399823+00:00","id":6392,"links":{},"metadata":{"_buckets":{"deposit":"8cbb047e-ef1e-466e-95ec-be6681508822"},"_deposit":{"created_by":3,"id":"6392","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"6392"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00006392","sets":["9:27:28:29"]},"author_link":["20433","20430","20431","4493","19480","20432"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-07","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"479-9","bibliographicPageStart":"479-1","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"Frontiers in Endocrinology"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Extra-adrenal steroid hormone production has been reported in several tissues, the biological role of which is interesting in terms of hormonal regulation of metabolism, growth, and behavior. In this report, we describe for the first time steroidogenesis in rat salivary glands. Enzyme activities associated with corticosterone and testosterone production were detected in rat salivary glands by LC-MS analysis. In tissue homogenates of rat salivary glands, progesterone was produced enzymatically in vitro from pregnenolone in the presence of NADPH and NADH. Deoxycorticosterone was produced from progesterone, corticosterone from deoxycorticosterone, and testosterone from androstenedione (but not pregnenolone from cholesterol) via enzymatic reactions using the same tissue homogenates. Immunoblotting analysis indicated the expression of 11β-hydroxylase (cytochrome P450 11β1; CYP11β1), which mediated the production of corticosterone from deoxycorticosterone. However, CYP family 11 subfamily A member 1 (CYP11A1)-mediated production of pregnenolone from cholesterol was not detected in the salivary glands by immunoblotting using a specific antibody. These results indicate that corticosterone and testosterone are produced from pregnenolone in rat salivary glands. The initial substrate in salivary steroidogenesis and the roles of salivary corticosterone and testosterone are discussed.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Frontiers Media"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3389/fendo.2019.00479","subitem_relation_type_select":"DOI"}}]},"item_2_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2019 Ieko, Sasaki, Maeda, Fujiki, Iwano and Yokota. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms."}]},"item_2_rights_16":{"attribute_name":"権利（URI）","attribute_value_mlt":[{"subitem_rights":"http://creativecommons.org/licenses/by/4.0/ | http://creativecommons.org/licenses/by/4.0/"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ieko, Takahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sasaki, Hirokuni"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Maeda, Naoyuki","creatorNameLang":"en"},{"creatorName":"前田, 尚之","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Fujiki, Jumpei","creatorNameLang":"en"},{"creatorName":"藤木, 純平","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"岩野, 英知","creatorNameLang":""}],"familyNames":[{"familyNameLang":""}],"givenNames":[{"givenNameLang":""}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Yokota, Hiroshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-18"}],"displaytype":"detail","filename":"R-2020-45_fujiki.pdf","filesize":[{"value":"2.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"R-2020-45_fujiki.pdf","url":"https://rakuno.repo.nii.ac.jp/record/6392/files/R-2020-45_fujiki.pdf"},"version_id":"7e2d1370-ccb6-45de-a636-f5d92a024626"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"salivary glands","subitem_subject_scheme":"Other"},{"subitem_subject":"steroidogenesis","subitem_subject_scheme":"Other"},{"subitem_subject":"corticosterone","subitem_subject_scheme":"Other"},{"subitem_subject":"testosterone","subitem_subject_scheme":"Other"},{"subitem_subject":"steroid","subitem_subject_scheme":"Other"},{"subitem_subject":"pregnenolone","subitem_subject_scheme":"Other"},{"subitem_subject":"sulfate","subitem_subject_scheme":"Other"},{"subitem_subject":"conjugate","subitem_subject_scheme":"Other"},{"subitem_subject":"salivary glands","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"steroidogenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"corticosterone","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"testosterone","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"steroid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"pregnenolone","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sulfate","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"conjugate","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Analysis of Corticosterone and Testosterone Synthesis in Rat Salivary Gland Homogenates","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Analysis of Corticosterone and Testosterone Synthesis in Rat Salivary Gland Homogenates"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-11-18"},"publish_date":"2020-11-18","publish_status":"0","recid":"6392","relation_version_is_last":true,"title":["Analysis of Corticosterone and Testosterone Synthesis in Rat Salivary Gland Homogenates"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-31T02:14:50.568074+00:00"}