{"created":"2023-06-19T07:03:54.832350+00:00","id":6402,"links":{},"metadata":{"_buckets":{"deposit":"c6ef3a7d-d931-4b01-85a5-16846a6522e4"},"_deposit":{"created_by":3,"id":"6402","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"6402"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00006402","sets":["9:27:28:29"]},"author_link":["20474","4758","20476","20477","20475","7686","4749"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"192","bibliographicPageStart":"188","bibliographicVolumeNumber":"82","bibliographic_titles":[{"bibliographic_title":"Journal of Veterinary Medical Science"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"East Coast fever is caused by Theileria parva, and poses serious concerns for dairy farmers owing to massive economic losses. In the current study, we compared three methods (DNA extraction kits, FTA-NaOH and FTA-TENT) of DNA extraction to identify the most economical and reliable method. A survey for T. parva prevalence was conducted in dairy cattle in Mbarara, Uganda. Cytochrome C oxidase subunit I (COI) and T. parva-p104 genes were amplified to compare the methods. FTA-NaOH-based polymerase chain reaction (PCR) yielded the best detection rate for both COI gene and p104 gene. Prevalence of T. parva was 45.0% and 83.3% at animal and farm-level, respectively. FTA-NaOH based-PCR is simple, highly sensitive and cost-effective tool for T. parva diagnosis in resource constrained settings.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本獣医学会"}]},"item_2_relation_11":{"attribute_name":"ISBN","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"0916-7250","subitem_relation_type_select":"ISBN"}}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1292/jvms.19-0521","subitem_relation_type_select":"DOI"}}]},"item_2_rights_16":{"attribute_name":"権利（URI）","attribute_value_mlt":[{"subitem_rights":"https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"内田, 玲麻","creatorNameLang":""}],"familyNames":[{"familyNameLang":""}],"givenNames":[{"givenNameLang":""}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"BYARUHANGA, Joseph"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OKAMURA, Ikuo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MIYAMA, Takeshi"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"MURAMATSU, Yasukazu","creatorNameLang":"en"},{"creatorName":"村松, 康和","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"VUDRIKO, Patrick"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"MAKITA, Kohei","creatorNameLang":"en"},{"creatorName":"蒔田, 浩平","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-11-18"}],"displaytype":"detail","filename":"R-2020-60_makita.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"R-2020-60_makita.pdf","url":"https://rakuno.repo.nii.ac.jp/record/6402/files/R-2020-60_makita.pdf"},"version_id":"e30f3f9a-08e3-4978-bc24-bf38782b877f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"dairy cow","subitem_subject_scheme":"Other"},{"subitem_subject":"East Coast fever","subitem_subject_scheme":"Other"},{"subitem_subject":"FTA card","subitem_subject_scheme":"Other"},{"subitem_subject":"sodium hydroxide","subitem_subject_scheme":"Other"},{"subitem_subject":"Theileria parva","subitem_subject_scheme":"Other"},{"subitem_subject":"dairy cow","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"East Coast fever","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"FTA card","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sodium hydroxide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Theileria parva","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"FTA-Sodium hydroxide-based polymerase chain reaction (PCR) : an efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"FTA-Sodium hydroxide-based polymerase chain reaction (PCR) : an efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-11-18"},"publish_date":"2020-11-18","publish_status":"0","recid":"6402","relation_version_is_last":true,"title":["FTA-Sodium hydroxide-based polymerase chain reaction (PCR) : an efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-31T02:26:24.543420+00:00"}