@article{oai:rakuno.repo.nii.ac.jp:00006768,
 author = {NAGAHATA, Hajime and MORIYAMA, Ayumi and SAWADA, Chika and ASAI, Yukiko and KOKUBU, Chihiro and Gondaira, Satoshi and 権平, 智 and 樋口, 豪紀},
 issue = {12},
 journal = {Journal of Veterinary Medical Science},
 month = {Dec},
 note = {Article, This study aimed to evaluate innate immune responses of mammary glands induced by intramammary infusion of Bifidobacterium breve in dairy cows. Somatic cell counts in quarters of cows showed a marked increase following B. breve infusion on days 1 and 2. Opsonizedstimulated chemiluminescence response in quarter milk was significantly (P<0.05) increased by B. breve infusion on days 1 to 3 compared to that of pre-infusion. Lactoferrin concentrations in B. breve-infused quarter milk increased significantly (P<0.05) on days 2 to 4 and 6 compared to those of pre-infusion. IgG and IgA concentrations in B. breve-infused quarters significantly (P<0.05) increased on days 2 to 4 for IgG and days 3, 4, 6 and 8 for IgA compared to those of pre-infusion. Interleukin (IL)-1β and IL-8 mRNA levels in somatic cells from B. breve-infused quarters were significantly (P<0.05) upregulated on day 1 compared to those on days 0 and 14. Conversely, IL-6 mRNA levels in somatic cells from B. breve-infused quarters on days 0, 1 and 14 and NF-κB mRNA levels on day 0 were significantly (P<0.05) down-regulated compared to those of control. IL-1β, tumor necrosis factor (TNF)-α and IL-6 concentrations increased on days 1, 3 and 7 after B. breve infusion in quarters. Intramammary infusion of B. breve (3 × 109 cfu) induces a massive influx of leukocytes and enhances innate immune response in mammary glands. This event may contribute to the enhancing host defense in the mammary gland.},
 pages = {1742--1749},
 title = {Innate immune response of mammary gland induced by intramammary infusion of Bifidobacterium breve in lactating dairy cows},
 volume = {82},
 year = {2020}
}