{"created":"2023-06-19T07:04:13.926975+00:00","id":6812,"links":{},"metadata":{"_buckets":{"deposit":"21daaf20-0625-4e81-9ad3-70948ebc566a"},"_deposit":{"created_by":3,"id":"6812","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"6812"},"status":"published"},"_oai":{"id":"oai:rakuno.repo.nii.ac.jp:00006812","sets":["9:27:28:29"]},"author_link":["22132","21863","22133","22128","22136","22138","22125","22137","22134","22122","22118","22135","22126","22120","22131","22124","22123","22127","4494","22130","22121","4871","22119","22129"],"item_2_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-02","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"12-14","bibliographicPageStart":"12-1","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"Frontiers in Veterinary Science"}]}]},"item_2_description_43":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Article","subitem_description_type":"Other"}]},"item_2_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Bovine mycoplasmosis caused by Mycoplasma bovis results in pneumonia and mastitis in cattle. We previously demonstrated that the programmed death_1 (PD_<-1>)/PD-ligand _1 (PD-L_1) pathway is involved in immune dysfunction during M. bovis infection and that prostaglandin E_2 (PGE_2) suppressed immune responses and upregulated PD-L_1 expression in Johne's disease, a bacterial infection in cattle. In this study, we investigated the role of PGE_2 in immune dysfunction and the relationship between PGE_2 and the PD-1/PD-L1 pathway in M. bovis infection. In vitro stimulation with M. bovis upregulated the expressions of PGE_2 and PD-L_1 presumably via Toll-like receptor 2 in bovine peripheral blood mononuclear cells (PBMCs). PGE_2 levels of peripheral blood in infected cattle were significantly increased compared with those in uninfected cattle. Remarkably, plasma PGE_2 levels were positively correlated with the proportions of PD-L_1^+ monocytes in M. bovis-infected cattle. Additionally, plasma PGE_2 production in infected cattle was negatively correlated with M. bovis-specific interferon (IFN)-γ production from PBMCs. These results suggest that PGE_2 could be one of the inducers of PD-L1 expression and could be involved in immunosuppression during M. bovis infection. In vitro blockade assays using anti-bovine PD-L_1 antibody and a cyclooxygenase 2 inhibitor significantly upregulated the M. bovis-specific IFN-γ response. Our study findings might contribute to the development of novel therapeutic strategies for bovine mycoplasmosis that target PGE_2 and the PD_<-1>/PD-L_1 pathway.","subitem_description_type":"Abstract"}]},"item_2_publisher_36":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Frontiers Media SA"}]},"item_2_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.3389/fvets.2020.00012","subitem_relation_type_select":"DOI"}}]},"item_2_rights_16":{"attribute_name":"権利（URI）","attribute_value_mlt":[{"subitem_rights":"http://creativecommons.org/licenses/by/4.0/ | http://creativecommons.org/licenses/by/4.0/"}]},"item_2_version_type_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Goto, Shinya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Konnai, Satoru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirano, Yuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kohara, Junko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okagawa, Tomohiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Maekawa, Naoya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sajiki, Yamato"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watari, Kei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Minato, Erina"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Atsuhi"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"Gondaira, Satoshi","creatorNameLang":"en"},{"creatorName":"権平, 智","creatorNameLang":"ja"}],"familyNames":[{},{}],"givenNames":[{},{}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"樋口, 豪紀","creatorNameLang":""}],"familyNames":[{"familyNameLang":""}],"givenNames":[{"givenNameLang":""}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Koiwa, Masateru"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"田島, 誉士","creatorNameLang":""}],"familyNames":[{"familyNameLang":""}],"givenNames":[{"givenNameLang":""}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Taguchi, Eiji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Uemura, Ryoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamada, Shinji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kaneko, Mika K."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Yukinari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, Keiichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Toda, Mikihiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Yasuhiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Murata, Shiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohashi, Kazuhiko"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-02-21"}],"displaytype":"detail","filename":"R-2021-208_higuchi.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"R-2021-208_higuchi.pdf","url":"https://rakuno.repo.nii.ac.jp/record/6812/files/R-2021-208_higuchi.pdf"},"version_id":"6d3a587b-429b-4aa0-a161-20d5172f7e93"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"immunoinhibitory molecules","subitem_subject_scheme":"Other"},{"subitem_subject":"PD-1","subitem_subject_scheme":"Other"},{"subitem_subject":"PD-L1","subitem_subject_scheme":"Other"},{"subitem_subject":"T-cell exhaustion","subitem_subject_scheme":"Other"},{"subitem_subject":"prostaglandin E_2","subitem_subject_scheme":"Other"},{"subitem_subject":"immune dysfunction","subitem_subject_scheme":"Other"},{"subitem_subject":"Mycoplasma bovis","subitem_subject_scheme":"Other"},{"subitem_subject":"cattle","subitem_subject_scheme":"Other"},{"subitem_subject":"immunoinhibitory molecules","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PD-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PD-L1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"T-cell exhaustion","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"prostaglandin E_2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"immune dysfunction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mycoplasma bovis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cattle","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Upregulation of PD-L1 Expression by Prostaglandin E_2 and the Enhancement of IFN-γ by Anti-PD-L1 Antibody Combined With a COX-2 Inhibitor in Mycoplasma bovis Infection","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Upregulation of PD-L1 Expression by Prostaglandin E_2 and the Enhancement of IFN-γ by Anti-PD-L1 Antibody Combined With a COX-2 Inhibitor in Mycoplasma bovis Infection"}]},"item_type_id":"2","owner":"3","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-02-21"},"publish_date":"2022-02-21","publish_status":"0","recid":"6812","relation_version_is_last":true,"title":["Upregulation of PD-L1 Expression by Prostaglandin E_2 and the Enhancement of IFN-γ by Anti-PD-L1 Antibody Combined With a COX-2 Inhibitor in Mycoplasma bovis Infection"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-31T02:07:28.502958+00:00"}