@article{oai:rakuno.repo.nii.ac.jp:00007159,
 author = {HUDAGULA and 南, 典子 and 髙橋, 宗一郎 and 吉田, 訓子 and 近藤, 良介 and 山口, 昭弘},
 issue = {7},
 journal = {日本食品科学工学会誌},
 month = {},
 note = {Article, 自然発酵ワイン沈殿物，本学キャンパスおよび市販の植物材料から野生酵母225株を分離した．この中から選抜した59株を用いブドウ灰色カビ病の病原菌Botrytis cinereaに対する抗カビ作用をin vitroで評価したところ，強い抗カビ作用を示す20株が得られた．この内，最も多くの16株が得られたSaccharomyces cerevisiae について，遺伝子多型解析による株鑑別を行い，抗カビ作用を比較したところ，株型ごとに抗カビ作用の強さが異なることがわかった．このとき，S. cerevisiaeの多型解析には自動rRNA遺伝子間多型解析（ARISA）が従来の手法に比べ極めて有用性が高いことがわかった．, The antifungal activity against Botrytis cinerea, a pathogen of grape gray mold disease, was validated in vitro for 59 of 225 wild yeast strains isolated from naturally fermented wine samples (69 strains), our university campus plants (129 strains), and commercially available fruits (27 strains). Twenty strains showing strong antifungal activity were identified. The majority of the strains were Saccharomyces cerevisiae (16 of the 20 strains). The S. cerevisiae strains were differentiated by genetic polymorphism analyses with comparisons of their antifungal activities. As a result, it was clarified that each strain type showed different antifungal activity, and that automatic rRNA intergenic spacer analysis was extremely useful for the differentiation of S. cerevisiae strains when compared to other conventional polymerase chain reaction-based methods.},
 pages = {328--338},
 title = {ブドウ灰色カビ病に対する微生物農薬としてのヴィンヤード野生酵母の探索},
 volume = {68},
 year = {2021}
}