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Rapid detection of Salmonella enterica serovar Typhimurium DT104 strains by the polymerase chain reaction

http://hdl.handle.net/10659/5001
http://hdl.handle.net/10659/5001
1488cf02-48e8-4e21-8510-481bedfb4d5a
名前 / ファイル ライセンス アクション
S-2016-135_tamura.pdf S-2016-135_tamura.pdf (984.3 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2017-06-09
タイトル
タイトル Rapid detection of Salmonella enterica serovar Typhimurium DT104 strains by the polymerase chain reaction
言語
言語 eng
キーワード
主題Scheme Other
主題 Detection
キーワード
主題Scheme Other
主題 Epidemiologic investigation
キーワード
主題Scheme Other
主題 PCR
キーワード
主題Scheme Other
主題 Salmonella Typhimurium DT104
キーワード
主題Scheme Other
主題 ST104
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
著者 Yukawa, Shoichiro

× Yukawa, Shoichiro

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Yukawa, Shoichiro

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Tamura, Yutaka

× Tamura, Yutaka

WEKO 7004

Tamura, Yutaka

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Tanaka, Kiyoshi

× Tanaka, Kiyoshi

WEKO 7005

Tanaka, Kiyoshi

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Uchida, Ikuo

× Uchida, Ikuo

WEKO 7006

Uchida, Ikuo

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抄録
内容記述タイプ Abstract
内容記述 [Background] Infection with Salmonella enterica is a major public health concern in developed countries, and multidrug-resistant strains have become increasingly prevalent. S. enterica serovar Typhimurium DT104 (DT104) strains are prevalent in livestock in Japan and include numerous strains of multidrug-resistant S. enterica. Epidemiological analysis of these strains is critical for both agriculture and public health; however, diagnostic tests for these strains have yielded inconsistent results [Results] We developed a rapid, simple, and inexpensive polymerase chain reaction test to detect multi-drug resistant DT104 strains. We designed primers specific to the prophage ST104 sequence encoded by DT104 strains and assessed the specificity of these primers by assaying a panel of 50 S. enterica isolates. Amplification products of the expected size were generated from the genomes of each of the DT104 strains; however, the ST104 primers failed to amplify products from non-DT104 strains of S. enterica serovar Typhimurium or other S. enterica serovars. Furthermore, a probe generated using the ST104 primers detected a restriction fragment encoding the ST104 region of DT104 by Southern hybridization. [Conclusions] The ST104 primers exhibit specificity to DT104 strains and are suitable for epidemiological applications.
書誌情報 Acta Veterinaria Scandinavia

巻 57, p. 59-1-59-5, 発行日 2015-09
ISSN
収録物識別子タイプ ISSN
収録物識別子 1751-0147
DOI
関連タイプ isIdenticalTo
識別子タイプ DOI
関連識別子 10.1186/s13028-015-0143-x
権利
権利情報 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
権利(URI)
権利情報 http://creativecommons.org/licenses/by/4.0/ | http://creativecommons.org/licenses/by/4.0/
著者版フラグ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
出版者
出版者 BioMed Central Ltd
資源タイプ
内容記述タイプ Other
内容記述 Article
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