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Generation and validation of novel anti-bovine CD163 monoclonal antibodies ABM-1A9 and ABM-2D6
http://hdl.handle.net/10659/00006309
http://hdl.handle.net/10659/0000630998125059-23b7-4ff6-af20-13b4a054a3af
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
R-2019-45_kitamura.pdf (7.0 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-02-26 | |||||
| タイトル | ||||||
| タイトル | Generation and validation of novel anti-bovine CD163 monoclonal antibodies ABM-1A9 and ABM-2D6 | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | CD163 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | M2 macrophage | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Monoclonal antibody | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Inflammation | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Immunohistochemistry | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ELISA | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | CD163 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | M2 macrophage | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Monoclonal antibody | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Inflammation | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Immunohistochemistry | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | ELISA | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Shimamoto, Yoshinori
× Shimamoto, Yoshinori× Nio-Kobayashi, Junko× Watarai, Hiroshi× Nagano, Masashi× Saito, Natsuko× Takahashi, Eiki× 樋口, 豪紀× Kobayashi, Atsushi× Kimura, Takashi× 北村, 浩 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The scavenger receptor CD163 is widely used as a cell signature for alternatively active “M2” macrophages in mammals. In this study, we generated two monoclonal antibodies, ABM-1A9 and ABM-2D6, against the extracellular region of bovine CD163. Conventional Western blotting using the antibodies yielded immunoreactive bands of bovine CD163 at 130 and 150 kDa in non-reduced and reduced spleen lysates, respectively. The minimum limit of detectable concentration of both antibodies was relatively lower (5.0 ng/mL) than that of the anti-human CD163 monoclonal antibody AM–3 K (>1.0 μg/mL), which has been used previously for the detection of bovine CD163. An immunohistochemical study using formalin-fixed paraffin-embedded sections revealed that ABM-1A9 and ABM-2D6 clearly stained some Iba1^+ macrophages in the lymph nodes of cattle with mastitis. Moreover, the CD163-stained macrophages were frequently observed engulfing leukocytes. ELISA using ABM-2D6 distinguished levels of circulating soluble CD163 in healthy cattle (less than 16.9 pmol/mL) and cattle with mastitis (more than 33.7 pmol/mL). These new monoclonal antibodies can be used in the diagnosis and evaluation of inflammatory disease prognosis in cattle with immunohistological analyses and blood test applications. | |||||
| 書誌情報 |
Veterinary Immunology and Immunopathology 巻 198, p. 6-13, 発行日 2018-04 |
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| ISBN | ||||||
| 識別子タイプ | ISBN | |||||
| 関連識別子 | 0165-2427 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/j.vetimm.2018.02.004 | |||||
| 権利 | ||||||
| 権利情報 | © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license. | |||||
| 権利（URI） | ||||||
| 権利情報 | http://creativecommons.org/licenses/by-nc-nd/4.0/ | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 出版者 | ||||||
| 出版者 | Elsevier B.V. | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
