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アイテム
Analysis of Corticosterone and Testosterone Synthesis in Rat Salivary Gland Homogenates
http://hdl.handle.net/10659/00006625
http://hdl.handle.net/10659/0000662518d627b6-64b9-402d-b762-7119415ff68b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
R-2020-45_fujiki.pdf (2.4 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-11-18 | |||||
| タイトル | ||||||
| タイトル | Analysis of Corticosterone and Testosterone Synthesis in Rat Salivary Gland Homogenates | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | salivary glands | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | steroidogenesis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | corticosterone | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | testosterone | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | steroid | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | pregnenolone | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | sulfate | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | conjugate | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | salivary glands | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | steroidogenesis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | corticosterone | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | testosterone | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | steroid | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | pregnenolone | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | sulfate | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | conjugate | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Ieko, Takahiro
× Ieko, Takahiro× Sasaki, Hirokuni× 前田, 尚之× 藤木, 純平× 岩野, 英知× Yokota, Hiroshi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Extra-adrenal steroid hormone production has been reported in several tissues, the biological role of which is interesting in terms of hormonal regulation of metabolism, growth, and behavior. In this report, we describe for the first time steroidogenesis in rat salivary glands. Enzyme activities associated with corticosterone and testosterone production were detected in rat salivary glands by LC-MS analysis. In tissue homogenates of rat salivary glands, progesterone was produced enzymatically in vitro from pregnenolone in the presence of NADPH and NADH. Deoxycorticosterone was produced from progesterone, corticosterone from deoxycorticosterone, and testosterone from androstenedione (but not pregnenolone from cholesterol) via enzymatic reactions using the same tissue homogenates. Immunoblotting analysis indicated the expression of 11β-hydroxylase (cytochrome P450 11β1; CYP11β1), which mediated the production of corticosterone from deoxycorticosterone. However, CYP family 11 subfamily A member 1 (CYP11A1)-mediated production of pregnenolone from cholesterol was not detected in the salivary glands by immunoblotting using a specific antibody. These results indicate that corticosterone and testosterone are produced from pregnenolone in rat salivary glands. The initial substrate in salivary steroidogenesis and the roles of salivary corticosterone and testosterone are discussed. | |||||
| 書誌情報 |
Frontiers in Endocrinology 巻 10, p. 479-1-479-9, 発行日 2019-07 |
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| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.3389/fendo.2019.00479 | |||||
| 権利 | ||||||
| 権利情報 | © 2019 Ieko, Sasaki, Maeda, Fujiki, Iwano and Yokota. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | |||||
| 権利（URI） | ||||||
| 権利情報 | http://creativecommons.org/licenses/by/4.0/ | http://creativecommons.org/licenses/by/4.0/ | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | Frontiers Media | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
