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Placental transfer of conjugated bisphenol A and subsequent reactivation in the rat fetus
http://hdl.handle.net/10659/2862
http://hdl.handle.net/10659/28620216a0c7-db85-496b-815f-bb9ada4cc1a8
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
S-2011-113_Iwano.pdf (2.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2012-10-01 | |||||
| タイトル | ||||||
| タイトル | Placental transfer of conjugated bisphenol A and subsequent reactivation in the rat fetus | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | bisphenol A | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | endocrine disruptors | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | estrogenic compounds | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | metabolism | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | transplacental | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Nishikawa, Miyu
× Nishikawa, Miyu× Iwano, Hidetomo× Yanagisawa, Risa× Koike, Nanako× Inoue, Hiroki× Yokota, Hiroshi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background : Bisphenol A (BPA), a well-known endocrine disruptor, is highly glucuronidated in the liver, and the resultant BPA-glucuronide (BPA-GA) is excreted primarily into bile. However, in rodents, prenatal exposure to low doses of BPA can adversely affect the fetus, despite the efficient drug-metabolizing systems of the dams. The transport mechanisms of BPA from mother to fetus are unknown. Objectives : To test our hypothesis that BPA-GA—an inactive metabolite—is passed through the placenta to the fetus, where it affects the fetus after reactivation, we investigated the placental transfer of BPA-GA and reactivation to BPA in the fetus. Methods : After performing uterine perfusion with BPA-GA in pregnant rats, we examined the expression and localization of the placental transporters for drug metabolites in the perfusate by reverse-transcriptase polymerase chain reaction and immunohistochemistry. We also investigated the deconjugation of BPA-GA in the fetus and examined uridine 5′-diphospho-glucuronosyltransferase (UGT) activity toward BPA and the expression of UGT isoforms in fetal liver. Results : We detected BPA-GA and deconjugated BPA in the fetus and amniotic fluid after perfusion. In the trophoblast cells, organic anion-transporting polypeptide 4a1 (Oatp4a1) was localized on the apical membrane, and multidrug resistance-associated protein 1 (Mrp1) was localized to the basolateral membrane. We observed deconjugation of BPA-GA in the fetus; furthermore, we found the expression of UGT2B1, which metabolizes BPA, to be quite low in the fetus. Conclusions : These results demonstrate that BPA-GA is transferred into the fetus and deconjugated in the fetus because of its vulnerable drug-metabolizing system. | |||||
| 書誌情報 |
Environmental Health Perspectives 巻 118, 号 9, p. 1196-1203, 発行日 2010-09 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0091-6765 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1289/ehp.0901575 | |||||
| 権利 | ||||||
| 権利情報 | Reproduced with permission from Environmental Health Perspectives | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | Environmental Health Perspectives | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
