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Rapid detection of Salmonella enterica serovar Typhimurium DT104 strains by the polymerase chain reaction
http://hdl.handle.net/10659/5001
http://hdl.handle.net/10659/50011488cf02-48e8-4e21-8510-481bedfb4d5a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
S-2016-135_tamura.pdf (984.3 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-06-09 | |||||
| タイトル | ||||||
| タイトル | Rapid detection of Salmonella enterica serovar Typhimurium DT104 strains by the polymerase chain reaction | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Detection | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Epidemiologic investigation | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PCR | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Salmonella Typhimurium DT104 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ST104 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Yukawa, Shoichiro
× Yukawa, Shoichiro× Tamura, Yutaka× Tanaka, Kiyoshi× Uchida, Ikuo |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | [Background] Infection with Salmonella enterica is a major public health concern in developed countries, and multidrug-resistant strains have become increasingly prevalent. S. enterica serovar Typhimurium DT104 (DT104) strains are prevalent in livestock in Japan and include numerous strains of multidrug-resistant S. enterica. Epidemiological analysis of these strains is critical for both agriculture and public health; however, diagnostic tests for these strains have yielded inconsistent results [Results] We developed a rapid, simple, and inexpensive polymerase chain reaction test to detect multi-drug resistant DT104 strains. We designed primers specific to the prophage ST104 sequence encoded by DT104 strains and assessed the specificity of these primers by assaying a panel of 50 S. enterica isolates. Amplification products of the expected size were generated from the genomes of each of the DT104 strains; however, the ST104 primers failed to amplify products from non-DT104 strains of S. enterica serovar Typhimurium or other S. enterica serovars. Furthermore, a probe generated using the ST104 primers detected a restriction fragment encoding the ST104 region of DT104 by Southern hybridization. [Conclusions] The ST104 primers exhibit specificity to DT104 strains and are suitable for epidemiological applications. | |||||
| 書誌情報 |
Acta Veterinaria Scandinavia 巻 57, p. 59-1-59-5, 発行日 2015-09 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1751-0147 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1186/s13028-015-0143-x | |||||
| 権利 | ||||||
| 権利情報 | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | |||||
| 権利（URI） | ||||||
| 権利情報 | http://creativecommons.org/licenses/by/4.0/ | http://creativecommons.org/licenses/by/4.0/ | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | BioMed Central Ltd | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
