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TNFα-mediated plasminogen activation on neutrophils is involved in the high plasmin activity in mammary secretion of drying-off cows
http://hdl.handle.net/10659/1801
http://hdl.handle.net/10659/18018b36d5e9-12cc-4b5a-bcf7-9b832b580a8b
名前 / ファイル | ライセンス | アクション |
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S-735_Nagahata.pdf (310.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2011-03-31 | |||||
タイトル | ||||||
タイトル | TNFα-mediated plasminogen activation on neutrophils is involved in the high plasmin activity in mammary secretion of drying-off cows | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Chou, Wen K
× Chou, Wen K× Yu, Ting C× Chen, Shuen E× Peh, Ho C× Liu, Wen B× Chen, Ming T× Nagahata, Hajime× Chang, Chai J |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Interactions between inflammatory cytokines and plasminogen (Pg) activation system on immune cells are yet to be established. In previous studies we reported a somatic cell-associated elevation of proteolytic activity in mammary secretion of drying-off goats and cows. The purposes of the present study were to examine the role of TNF-α in polymorphonuclear neutrophil (PMN)-associated Pg activation, and the significance of this activation pathway for overall plasmin (Pm) activity in mammary secretion of drying-off cows. Results of experiments in vitro showed that the spontaneous Pg activation observed on fresh preparations of bovine blood PMN was completely blocked by anti bovine TNF-α antibody, and was further up-regulated by exogenous bovine TNF-α. Monitoring the parameters of mammary secretion of drying-off cows revealed that both somatic cell counts and differential PMN ratio was significantly elevated at weeks 1, 2 and 3 of milk stasis. Nevertheless, specific activity of soluble Pm in mammary secretion increased and the level of 17-kDa TNF-α decreased immediately following milk stasis. Iimmunoblotting revealed that although both 26-kDa pro-TNF-α and 17-kDa TNF-α were consistently present in somatic cells of mammary secretion collected at weeks 0, 1, 2 and 3 of milk stasis, only 26-kDa pro-TNF-α was present in somatic cells of milk during lactation. In-vitro assay indicated that cell-free mammary secretion of drying-off cows exerted no Pg activation bioactivity towards bovine blood PMN. Altogether, the current study suggests the existence of an active TNF-α-Pg-Pm autocrine/paracrine loop on the massively infiltrated PMN inside udders of drying-off cows, which involves extensive binding and internalization of 17-kDa TNF-α on PMN and consequently activation of Pg, resulting in high Pm activity and low 17-kDa TNF-α level in mammary secretion. These coordinated mechanisms may play a role in the defence of drying-off mammary gland. | |||||
書誌情報 |
Journal of Dairy Research 巻 76, 号 4, p. 459-468, 発行日 2009-11 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0022-0299 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1017/S0022029909990069 | |||||
権利 | ||||||
権利情報 | © Proprietors of Journal of Dairy Research 2009 | |||||
権利(URI) | ||||||
権利情報 | http://journals.cambridge.org/action/displayJournal?jid=DAR | http://journals.cambridge.org/action/displayJournal?jid=DAR | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | Cambridge University Press | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |