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Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1→6)-α-nigerosyl
http://hdl.handle.net/10659/00005419
http://hdl.handle.net/10659/000054192fcf63a5-1a30-4d51-9f42-69eb36fd07bd
名前 / ファイル | ライセンス | アクション |
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R-2017-145_Iwasaki.pdf (1.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-04-12 | |||||
タイトル | ||||||
タイトル | Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1→6)-α-nigerosyl | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Tagami, Takayoshi
× Tagami, Takayoshi× Miyano, Eri× Sadahiro, Juri× Okuyama, Masayuki× 岩﨑, 智仁× Kimura, Atsuo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The actinobacterium Kribbella flavida NBRC 14399^T produces cyclobis-(1→6)-α-nigerosyl (CNN), a cyclic glucotetraose with alternate α-(1→6)- and α-(1→3)-glucosidic linkages, from starch in the culture medium. We identified gene clusters associated with the production and intracellular catabolism of CNN in the K. flavida genome. One cluster encodes 6-α-glucosyltransferase and 3-α-isomaltosyltransferase, which are known to coproduce CNN from starch. The other cluster contains four genes annotated as a transcriptional regulator, sugar transporter, glycoside hydrolase family (GH) 31 protein (Kfla1895), and GH15 protein (Kfla1896). Kfla1895 hydrolyzed the α-(1→3)-glucosidic linkages of CNN and produced isomaltose via a possible linear tetrasaccharide. The initial rate of hydrolysis of CNN (11.6 s^<−1>) was much higher than that of panose (0.242 s^<−1>), and hydrolysis of isomaltotriose and nigerose was extremely low. Because Kfla1895 has a strong preference for the α-(1→3)-isomaltosyl moiety and effectively hydrolyzes the α-(1→3)-glucosidic linkage, it should be termed 1,3-α-isomaltosidase. Kfla1896 effectively hydrolyzed isomaltose with liberation of β-glucose, but displayed low or no activity toward CNN and the general GH15 enzyme substrates such as maltose, soluble starch, or dextran. The k_<cat>/K_m for isomaltose (4.81 ± 0.18^<s−1> mm^<−1>) was 6.9- and 19-fold higher than those for panose and isomaltotriose, respectively. These results indicate that Kfla1896 is a new GH15 enzyme with high substrate specificity for isomaltose, suggesting the enzyme should be designated an isomaltose glucohydrolase. This is the first report to identify a starch-utilization pathway that proceeds via CNN. | |||||
書誌情報 |
The Journal of Biological Chemistry 巻 291, 号 32, p. 16438-16447, 発行日 2016-08 |
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ISBN | ||||||
識別子タイプ | ISBN | |||||
関連識別子 | 1083-351X | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | http://dx.doi.org/10.1074/jbc.M116.727305 | |||||
権利 | ||||||
権利情報 | This research was originally published in The Journal of Biological Chemistry. Takayoshi Tagami, Eri Miyano, Juri Sadahiro, Masayuki Okuyama, Tomohito Iwasaki and Atsuo Kimura. Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1→6)-α-nigerosyl. The Journal of Biological Chemistry. 2016; 291(32): 16438-16447. © the American Society for Biochemistry and Molecular Biology. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | American Society for Biochemistry and Molecular Biology | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |