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Chromosome Doubling in Limonium bellidifolium (Gouan) Dumort. by Colchicine Treatment of Seeds
http://hdl.handle.net/10659/00005424
http://hdl.handle.net/10659/000054240305948c-b743-40b5-956b-f19e15e08eb6
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
R-2017-172_Mori.pdf (3.5 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2018-04-12 | |||||
| タイトル | ||||||
| タイトル | Chromosome Doubling in Limonium bellidifolium (Gouan) Dumort. by Colchicine Treatment of Seeds | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | antimitotic agent | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | flow cytometry | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | perennial statice | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | polyploidization | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | tetraploid | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
森, 志郎
× 森, 志郎× Yamane, Takuya× Yahata, Masaki× Shinoda, Koichi× Murata, Naho |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Limonium bellidifolium (2n = 2x = 18), a perennial statice belonging to the family Plumbaginaceae, is cultivated as a garden plant or for cut flowers and is an important breeding material for the production of hybrid cultivars in the genus. In this study, chromosome doubling in L. bellidifolium was attempted to increase the variability in horticultural traits. Seeds of this species were treated with an antimitotic agent, colchicine, at different concentrations and exposure periods. The treated seeds were sown in soil in a cell tray and the seedlings were grown in a greenhouse. More than 50% of the seedlings treated with colchicine for 24 or 48 h, irrespective of the concentration, survived for 4 months after treatment. Most of the seedlings treated for 72 h at any concentration showed very poor growth and abnormal thickening of the hypocotyl, and ultimately died. The surviving seedlings were grown in 9-cm pots. Flow cytometry analysis of leaf tissues showed that 2.5%–5% of plants that received 0.05% colchicine for 72 h, 0.25% colchicine for 24 h, 0.25% colchicine for 48 h, or 0.5% colchicine for 48 h were tetraploid (4x) or mixoploid (2x + 4x). The highest frequencies of tetraploids and mixoploids occurred following treatment with 0.05% colchicine for 72 h. These results showed that colchicine treatment of seeds is effective for chromosome doubling in L. bellidifolium. After 3 years of cultivation, the morphological characteristics of diploid and tetraploid L. bellidifolium plants at the flowering stage were investigated. The stomatal density was lower in all investigated tetraploid and mixoploid plants than in the control diploid plant. The stomatal length was 1.1- to 1.5-fold higher in all tetraploid and mixoploid plants than in the control. Tetraploid plants tended to have wider and thicker leaves than the control and also produced larger flowers. | |||||
| 書誌情報 |
Horticulture Journal 巻 85, 号 4, p. 366-371, 発行日 2016-01 |
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| ISBN | ||||||
| 識別子タイプ | ISBN | |||||
| 関連識別子 | 2189-0102 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://dx.doi.org/10.2503/hortj.MI-117 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 園芸学会 (The Japanese Society for Horticultural Science) | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
