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FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda
http://hdl.handle.net/10659/00007041
http://hdl.handle.net/10659/000070410a2a8f31-ab03-4011-9730-465c328f1341
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
R-2021-197_makita.pdf (2.0 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-02-21 | |||||
| タイトル | ||||||
| タイトル | FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | dairy cow | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | East Coast fever | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | FTA card, sodium hydroxide | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Theileria parva | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | dairy cow | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | East Coast fever | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | FTA card, sodium hydroxide | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Theileria parva | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
内田, 玲麻
× 内田, 玲麻× BYARUHANGA, Joseph× OKAMURA, Ikuo× MIYAMA, Takeshi× 村松, 康和× VUDRIKO, Patrick× 蒔田, 浩平 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | East Coast fever is caused by Theileria parva, and poses serious concerns for dairy farmers owing to massive economic losses. In the current study, we compared three methods (DNA extraction kits, FTA-NaOH and FTA-TENT) of DNA extraction to identify the most economical and reliable method. A survey for T. parva prevalence was conducted in dairy cattle in Mbarara, Uganda. Cytochrome C oxidase subunit I (COI) and T. parva-p104 genes were amplified to compare the methods. FTA-NaOH-based polymerase chain reaction (PCR) yielded the best detection rate for both COI gene and p104 gene. Prevalence of T. parva was 45.0% and 83.3% at animal and farmlevel, respectively. FTA-NaOH based-PCR is simple, highly sensitive and cost-effective tool for T. parva diagnosis in resource constrained settings. | |||||
| 書誌情報 |
Journal of Veterinary Medical Science 巻 82, 号 2, p. 188-192, 発行日 2020-02 |
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| ISBN | ||||||
| 識別子タイプ | ISBN | |||||
| 関連識別子 | 0916-7250 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1292/jvms.19-0521 | |||||
| 権利（URI） | ||||||
| 権利情報 | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 日本獣医学会 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
