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Structural determination, distribution, and physiological actions of ghrelin in the guinea pig
http://hdl.handle.net/10659/00006343
http://hdl.handle.net/10659/0000634374fc7e1c-f496-4df7-b419-3618187bb40b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
R-2019-143_kitazawa.pdf (2.4 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-02-26 | |||||
| タイトル | ||||||
| タイトル | Structural determination, distribution, and physiological actions of ghrelin in the guinea pig | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Guinea pig | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Ghrelin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Growth hormone secretagogue receptor 1a (GHS-R1a) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cDNA cloning | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Functional characterization | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Tissue distribution | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Guinea pig | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Ghrelin | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Growth hormone secretagogue receptor 1a (GHS-R1a) | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | cDNA cloning | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Functional characterization | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Tissue distribution | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Okuhara, Yuji
× Okuhara, Yuji× Kaiya, Hiroyuki× TERAOKA, Hiroki× Kitazawa, Takio |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We identified guinea pig ghrelin (gp-ghrelin), and examined its distribution and physiological actions in the guinea-pig. Gp-ghrelin is a 28-amino acid peptide (GASFR SPEHH SAQQR KESRK LPAKI QPR); seven amino acids are different from that of rat ghrelin at positions 2, 5, 10, 11, 19, 21, and 25, which include the conserved region known in mammals. The third serine residue is mainly modified by n-decanoyl acid. Both gp-ghrelin and rat ghrelin increased intracellular Ca^<2+> concentration of HEK293 cells expressing guinea pig growth hormone secretagogue receptor 1a (GHS-R1a), and the affinity of gp-ghrelin was slightly higher than that of rat ghrelin. In addition, gp-ghrelin was also effective in CHO cells expressing rat GHS-R1a with similar affinity to that of rat ghrelin. Gp-ghrelin mRNA was predominantly expressed in the stomach, whereas the expression levels in other organs was low. High levels of GHS-R1a mRNA expression were observed in the pituitary, medulla oblongata, and kidney, while medium levels were noted in the thalamus, pons, olfactory bulb, and heart. Immunohistochemistry identified gp-ghrelin-immunopositive cells in the gastric mucosa and pancreas. Intraperitoneal injection of gp-ghrelin increased food intake in the guinea pig. Gp-ghrelin did not cause any mechanical responses in isolated gastrointestinal smooth muscles in vitro, similar to rat ghrelin. In conclusion, the N-terminal structures that are conserved in mammals were different in gp-ghrelin. Moreover, the functional characteristics of gp-ghrelin, other than its distribution, were dissimilar from those in other Rodentia. | |||||
| 書誌情報 |
Peptides 巻 99, p. 70-81, 発行日 2018-01 |
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| ISBN | ||||||
| 識別子タイプ | ISBN | |||||
| 関連識別子 | 0196-9781 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/j.peptides.2017.11.010 | |||||
| 権利 | ||||||
| 権利情報 | © 2017 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license. | |||||
| 権利（URI） | ||||||
| 権利情報 | http://creativecommons.org/licenses/by-nc-nd/4.0/ | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 出版者 | ||||||
| 出版者 | Elsevier Inc. | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
