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道央圏に自生するキノコの同定と機能性成分の含有量
http://hdl.handle.net/10659/4940
http://hdl.handle.net/10659/49401a4fffbf-aaa9-488e-bca3-5cd2a507e159
名前 / ファイル | ライセンス | アクション |
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S-2016-077_hoshiba.pdf (1.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-06-09 | |||||
タイトル | ||||||
タイトル | 道央圏に自生するキノコの同定と機能性成分の含有量 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | wildmushroom | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | species identification | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | genetic analysis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | beta-glucan | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | foodfunction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 野生キノコ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 品種鑑定 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 遺伝子解析 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | β-グルカン | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 機能性 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
菅原, 諒太
× 菅原, 諒太× 山田, さゆみ× 涂, 志豪× 菅原, 明子× 干場, 敏博× 山内, 正仁× 山口, 昭弘 |
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著者別名 | ||||||
姓名 | Sugawara, Ryota | |||||
著者別名 | ||||||
姓名 | Yamada, Sayumi | |||||
著者別名 | ||||||
姓名 | Tu, Zhihao | |||||
著者別名 | ||||||
姓名 | Sugawara, Akiko | |||||
著者別名 | ||||||
姓名 | Hoshiba, Toshihiro | |||||
著者別名 | ||||||
姓名 | Yamauchi, Masahito | |||||
著者別名 | ||||||
姓名 | Yamaguchi, Akihiro | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | This study focuses on the identification and characterization of wild mushrooms grown on the campus of Rakuno Gakuen University, using DNA sequencing and analysis of functional metabolites. Forty-eight samples of fruiting bodies were collected between June 2012 and November 2014. The samples were hot-air dried and powdered, and subsequently extracted with alkaline hot water to obtain beta-glucans, or 50% ethanol for total polyphenols (TPP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Functional metabolites were assessed by colorimetric assays, using Congo Red reagent for beta-glucans, Folin-Denis reagent for TPP level, and DPPH radical scavenging for antioxidant activity. Total DNA was extracted from each sample and subjected to DNA sequencing for the inter spacer regions of ribosomal RNA gene. Sequencing analysis identified 27 independent species or genus of mushrooms. Beta-glucan contents showed clear differences between species. The TPP levels and DPPH radical scavenging activity showed similar tendencies and were significantly correlated ( p<0.0001). 酪農学園大学キャンパス内の自生キノコ48試料について遺伝子解析による同定を行ったところ46試料が種あるいは属レベルで同定された.Congo Red法によるβ-グルカンの定量結果は,測定値にセルロースなどの直鎖状β-グルカンを含まないことから,酵素法と比べて小さい値となったがβ-グルカンの高次構造を反映した機能性を評価する上ではむしろ有用であると考えられた.また食材の機能性評価に汎用されるTPPおよびDPPHラジカル消去活性を測定したところ,両測定値間には強い正の相関が認められ,キノコの抗酸化性にはポリフェノールが関与していることが示唆された.β-グルカン,TPPおよびDPPHラジカル消去活性のいずれの機能性指標においても科ごとに一定の傾向は見られず,種ごとに特徴的な値を示すことが確認された. | |||||
書誌情報 |
日本食品科学工学会誌 巻 62, 号 9, p. 445-453, 発行日 2015-09 |
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DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3136/nskkk.62.445 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | Species Identification and Functional Components of Wild Mushrooms from Rakuno Gakuen University, Hokkaido | |||||
出版者 | ||||||
出版者 | 日本食品科学工学会 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article |