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Expression of the MDR1 gene and P-glycoprotein in canine mast cell tumor cell lines
http://hdl.handle.net/10659/1429
http://hdl.handle.net/10659/1429cbdca490-0bb7-4156-af48-30c0e910d74b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
S-172_Kadosawa.pdf (1.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2010-03-11 | |||||
| タイトル | ||||||
| タイトル | Expression of the MDR1 gene and P-glycoprotein in canine mast cell tumor cell lines | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
NAKAICHI, Munekazu
× NAKAICHI, Munekazu× TAKESHITA, Yoko× OKUDA, Masaru× NAKAMOTO, Yuya× ITAMOTO, Kazuhito× UNE, Satoshi× SASAKI, Nobuo× KADOSAWA, Tsuyoshi× TAKAHASHI, Tomoko× TAURA, Yasuho |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Cellular drug resistance to antineoplastic drugs is often due to the presence of a drug efflux pump that reduces intracellular drug accumulation and chemosensitivity. P-glycoprotein (P-gp), which is encoded by the MDR1 gene, is considered to function as an ATP-driven membrane drug efflux pump and appears to play an important role in tumor cell resistance. In the present report, we assessed the expression of MDR1 by RT-PCR in three canine mast cell tumor cell lines, TiMC, CoMS and LuMC, originating from a cutaneous tumor, an oral-mucosal tumor and a gastrointestinal tumor, respectively. P-gp expression was also examined by Western blot analysis, while the functional activity of P-gp was assessed by flowcytometric analysis of intracellular rhodamine-123 (Rhd-123) uptake. The results revealed that MDR1 gene and P-gp were both expressed in CoMS and LuMC cells, whereas neither was present in TiMC cells. In CoMS and LuMC cells, intracellular uptake of Rhd-123 increased in the presence of verapamil, a functional modulator of P-gp. In contrast, TiMC cells did not show any changes in the intracellular accumulation of Rhd-123 after the verapamil addition. These findings suggest that the expressions of MDR1 gene and P-gp probably contribute to cellular drug resistance in canine mast cell tumors. | |||||
| 書誌情報 |
The Journal of Veterinary Medical Science 巻 69, 号 2, p. 111-115, 発行日 2007-02 |
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| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0916-7250 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 日本獣医学会 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
