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Plasma endotoxin activity in kangaroos with oral necrobacillosis (lumpy jaw disease) using an automated handheld testing system
http://hdl.handle.net/10659/00005391
http://hdl.handle.net/10659/000053918311d0bf-f2a0-418a-88ba-76288c62a354
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
R-2017-74_Asakawa.pdf (906.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2018-04-12 | |||||
| タイトル | ||||||
| タイトル | Plasma endotoxin activity in kangaroos with oral necrobacillosis (lumpy jaw disease) using an automated handheld testing system | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
SOTOHIRA, Yukari
× SOTOHIRA, Yukari× SUZUKI, Kazuyuki× SASAKI, Haruka× SANO, Tadashi× TSUCHIYA, Masakazu× SUZUKI, Yohko× SHIMAMORI, Toshio× TSUKANO, Kenji× SATO, Ayano× YOKOTA, Hiroshi× 浅川, 満彦 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The aim of the present study was to evaluate the reliability and effectiveness of directly determining endotoxin activity in plasma samples from kangaroos with lumpy jaw disease (LJD, n=15) and healthy controls (n=12). Prior to the present study, the ability of the commercially available automated handheld portable test system (PTS^<TM>) to detect endotoxin activity in kangaroo plasma was compared with that of the traditional LAL-kinetic turbidimetric (KT) assay. Plasma samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTS^<TM> was not significantly different from that of the traditional LAL-based assay. The data obtained using PTS^<TM> correlated with those using KT (r^2=0.963, P<0.001). These findings indicated that the PTSTM is applicable as a simplified system to assess endotoxin activity in macropods. In the present study, we demonstrated the diagnostic value of plasma endotoxin activity in kangaroos with systemic inflammation caused by oral necrobacillosis and identified plasma endotoxin activity as a sensitive marker of systemic inflammation in kangaroos with LJD. Based on ROC curves, we proposed a diagnostic cut-off point for endotoxin activity of >0.22 EU/ml for the identification of LJD. Our results indicate that the assessment of plasma endotoxin activity is a promising diagnostic tool for determining the outcome of LJD in captive macropods. | |||||
| 書誌情報 |
The Journal of veterinary medical science 巻 78, 号 6, p. 971-976, 発行日 2016-06 |
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| ISBN | ||||||
| 識別子タイプ | ISBN | |||||
| 関連識別子 | 0916-7250 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://doi.org/10.1292/jvms.15-0513 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | 日本獣医学会 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
