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Efficacy of Recombinant Canine Distemper Virus Expressing Leishmania Antigen against Leishmania Challenge in Dogs
http://hdl.handle.net/10659/4955
http://hdl.handle.net/10659/49552349d81b-d94c-406c-af10-6ea0ca6d8e8e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
S-2016-177_kooriyama.pdf (3.8 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-06-09 | |||||
| タイトル | ||||||
| タイトル | Efficacy of Recombinant Canine Distemper Virus Expressing Leishmania Antigen against Leishmania Challenge in Dogs | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Miura, Ryuichi
× Miura, Ryuichi× Kooriyama, Takanori× Yoneda, Misako× Takenaka, Akiko× Doki, Miho× Goto, Yasuyuki× Sanjoba, Chizu× Endo, Yasuyuki× Fujiyuki, Tomoko× Sugai, Akihiro× Tsukiyama-Kohara, Kyoko× Matsumoto, Yoshitsugu× Sato, Hiroki× Kai, Chieko |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Canine distemper virus (CDV) vaccination confers long-term protection against CDV reinfection. To investigate the utility of CDV as a polyvalent vaccine vector for Leishmania, we generated recombinant CDVs, based on an avirulent Yanaka strain, that expressed Leishmania antigens: LACK, TSA, or LmSTI1 (rCDV–LACK, rCDV–TSA, and rCDV–LmSTI1, respectively). Dogs immunized with rCDV-LACK were protected against challenge with lethal doses of virulent CDV, in the same way as the parental Yanaka strain. To evaluate the protective effects of the recombinant CDVs against cutaneous leishmaniasis in dogs, dogs were immunized with one recombinant CDV or a cocktail of three recombinant CDVs, before intradermal challenge (in the ears) with infective-stage promastigotes of Leishmania major. Unvaccinated dogs showed increased nodules with ulcer formation after 3 weeks, whereas dogs immunized with rCDV–LACK showed markedly smaller nodules without ulceration. Although the rCDV–TSA- and rCDV–LmSTI1-immunized dogs showed little protection against L. major, the cocktail of three recombinant CDVs more effectively suppressed the progression of nodule formation than immunization with rCDV–LACK alone. These results indicate that recombinant CDV is suitable for use as a polyvalent live attenuated vaccine for protection against both CDV and L. major infections in dogs. | |||||
| 書誌情報 |
PLoS Neglected Tropical Diseases 巻 9, 号 7, p. e0003914-1-e0003914-15, 発行日 2015-07 |
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| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1371/journal.pntd.0003914 | |||||
| 権利 | ||||||
| 権利情報 | Copyright: © 2015 Miura et al. | |||||
| 権利 | ||||||
| 権利情報 | This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
| 権利（URI） | ||||||
| 権利情報 | https://creativecommons.org/licenses/by/4.0/ | https://creativecommons.org/licenses/by/4.0/ | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | Public Library of Science | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
